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phosphorylated p pparγ  (Bioss)


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    Structured Review

    Bioss phosphorylated p pparγ
    Effects of CB on the expression of macrophage polarization-related proteins. (A) Gel blots showing protein expression of Arg-1, iNOS, <t>p-PPARγ</t> and PPARγ in cells with different treatment groups. (B-D) Quantification of (B) Arg-1, (C) iNOS and (D) p-PPARγ based on the western blot assay (n=3). ** P<0.01 compared with M0 group; # P<0.05, ## P<0.01 and $$ P<0.01 compared with M2 group; ▲ P<0.05 and ▲▲ P<0.01 compared with M2 + RSG group. CB, cinobufagin; Arg-1, arginase 1; iNOS, inducible nitric oxide synthase; p-, <t>phosphorylated;</t> PPARγ, peroxisome proliferator-activated receptor γ; RSG, rosiglitazone; ns from M2 group, not statistically significant compared with M0 group; ns from M2 + RSG group, not statistically significant compared with M2 group.
    Phosphorylated P Pparγ, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+p+%E2%80%91ppar%CE%B3/PPAR+Gamma+(ser112)+Polyclonal+Antibody/pmc11419410-126-21-27
    Average 93 stars, based on 14 article reviews
    phosphorylated p pparγ - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Cinobufagin inhibits M2-like tumor-associated macrophage polarization to attenuate the invasion and migration of lung cancer cells"

    Article Title: Cinobufagin inhibits M2-like tumor-associated macrophage polarization to attenuate the invasion and migration of lung cancer cells

    Journal: International Journal of Oncology

    doi: 10.3892/ijo.2024.5690

    Effects of CB on the expression of macrophage polarization-related proteins. (A) Gel blots showing protein expression of Arg-1, iNOS, p-PPARγ and PPARγ in cells with different treatment groups. (B-D) Quantification of (B) Arg-1, (C) iNOS and (D) p-PPARγ based on the western blot assay (n=3). ** P<0.01 compared with M0 group; # P<0.05, ## P<0.01 and $$ P<0.01 compared with M2 group; ▲ P<0.05 and ▲▲ P<0.01 compared with M2 + RSG group. CB, cinobufagin; Arg-1, arginase 1; iNOS, inducible nitric oxide synthase; p-, phosphorylated; PPARγ, peroxisome proliferator-activated receptor γ; RSG, rosiglitazone; ns from M2 group, not statistically significant compared with M0 group; ns from M2 + RSG group, not statistically significant compared with M2 group.
    Figure Legend Snippet: Effects of CB on the expression of macrophage polarization-related proteins. (A) Gel blots showing protein expression of Arg-1, iNOS, p-PPARγ and PPARγ in cells with different treatment groups. (B-D) Quantification of (B) Arg-1, (C) iNOS and (D) p-PPARγ based on the western blot assay (n=3). ** P<0.01 compared with M0 group; # P<0.05, ## P<0.01 and $$ P<0.01 compared with M2 group; ▲ P<0.05 and ▲▲ P<0.01 compared with M2 + RSG group. CB, cinobufagin; Arg-1, arginase 1; iNOS, inducible nitric oxide synthase; p-, phosphorylated; PPARγ, peroxisome proliferator-activated receptor γ; RSG, rosiglitazone; ns from M2 group, not statistically significant compared with M0 group; ns from M2 + RSG group, not statistically significant compared with M2 group.

    Techniques Used: Expressing, Western Blot

    Related Articles

    Incubation:

    Article Title: Cinobufagin inhibits M2‑like tumor‑associated macrophage polarization to attenuate the invasion and migration of lung cancer cells.
    Article Snippet: Macrophages have crucial roles in immune responses and tumor progression, exhibiting diverse pheno‐ types based on environmental cues.. In the present study, the impact of cinobufagin (CB) on macrophage polarization and the consequences on tumor‐associated behaviors were investigated.. Morphological transformations of THP‐1 cells into M0, M1 and M2 macrophages were observed, including distinct changes in the size, shape and adherence properties of these cells.

    Membrane:

    Article Title: Cinobufagin inhibits M2‑like tumor‑associated macrophage polarization to attenuate the invasion and migration of lung cancer cells.
    Article Snippet: Macrophages have crucial roles in immune responses and tumor progression, exhibiting diverse pheno‐ types based on environmental cues.. In the present study, the impact of cinobufagin (CB) on macrophage polarization and the consequences on tumor‐associated behaviors were investigated.. Morphological transformations of THP‐1 cells into M0, M1 and M2 macrophages were observed, including distinct changes in the size, shape and adherence properties of these cells.

    Saline:

    Article Title: Cinobufagin inhibits M2‑like tumor‑associated macrophage polarization to attenuate the invasion and migration of lung cancer cells.
    Article Snippet: Macrophages have crucial roles in immune responses and tumor progression, exhibiting diverse pheno‐ types based on environmental cues.. In the present study, the impact of cinobufagin (CB) on macrophage polarization and the consequences on tumor‐associated behaviors were investigated.. Morphological transformations of THP‐1 cells into M0, M1 and M2 macrophages were observed, including distinct changes in the size, shape and adherence properties of these cells.

    Western Blot:

    Article Title: Cinobufagin inhibits M2‑like tumor‑associated macrophage polarization to attenuate the invasion and migration of lung cancer cells.
    Article Snippet: Macrophages have crucial roles in immune responses and tumor progression, exhibiting diverse pheno‐ types based on environmental cues.. In the present study, the impact of cinobufagin (CB) on macrophage polarization and the consequences on tumor‐associated behaviors were investigated.. Morphological transformations of THP‐1 cells into M0, M1 and M2 macrophages were observed, including distinct changes in the size, shape and adherence properties of these cells.



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    Effects of CB on the expression of macrophage polarization-related proteins. (A) Gel blots showing protein expression of Arg-1, iNOS, <t>p-PPARγ</t> and PPARγ in cells with different treatment groups. (B-D) Quantification of (B) Arg-1, (C) iNOS and (D) p-PPARγ based on the western blot assay (n=3). ** P<0.01 compared with M0 group; # P<0.05, ## P<0.01 and $$ P<0.01 compared with M2 group; ▲ P<0.05 and ▲▲ P<0.01 compared with M2 + RSG group. CB, cinobufagin; Arg-1, arginase 1; iNOS, inducible nitric oxide synthase; p-, <t>phosphorylated;</t> PPARγ, peroxisome proliferator-activated receptor γ; RSG, rosiglitazone; ns from M2 group, not statistically significant compared with M0 group; ns from M2 + RSG group, not statistically significant compared with M2 group.
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    Effects of CB on the expression of macrophage polarization-related proteins. (A) Gel blots showing protein expression of Arg-1, iNOS, <t>p-PPARγ</t> and PPARγ in cells with different treatment groups. (B-D) Quantification of (B) Arg-1, (C) iNOS and (D) p-PPARγ based on the western blot assay (n=3). ** P<0.01 compared with M0 group; # P<0.05, ## P<0.01 and $$ P<0.01 compared with M2 group; ▲ P<0.05 and ▲▲ P<0.01 compared with M2 + RSG group. CB, cinobufagin; Arg-1, arginase 1; iNOS, inducible nitric oxide synthase; p-, <t>phosphorylated;</t> PPARγ, peroxisome proliferator-activated receptor γ; RSG, rosiglitazone; ns from M2 group, not statistically significant compared with M0 group; ns from M2 + RSG group, not statistically significant compared with M2 group.
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    Image Search Results


    Effects of CB on the expression of macrophage polarization-related proteins. (A) Gel blots showing protein expression of Arg-1, iNOS, p-PPARγ and PPARγ in cells with different treatment groups. (B-D) Quantification of (B) Arg-1, (C) iNOS and (D) p-PPARγ based on the western blot assay (n=3). ** P<0.01 compared with M0 group; # P<0.05, ## P<0.01 and $$ P<0.01 compared with M2 group; ▲ P<0.05 and ▲▲ P<0.01 compared with M2 + RSG group. CB, cinobufagin; Arg-1, arginase 1; iNOS, inducible nitric oxide synthase; p-, phosphorylated; PPARγ, peroxisome proliferator-activated receptor γ; RSG, rosiglitazone; ns from M2 group, not statistically significant compared with M0 group; ns from M2 + RSG group, not statistically significant compared with M2 group.

    Journal: International Journal of Oncology

    Article Title: Cinobufagin inhibits M2-like tumor-associated macrophage polarization to attenuate the invasion and migration of lung cancer cells

    doi: 10.3892/ijo.2024.5690

    Figure Lengend Snippet: Effects of CB on the expression of macrophage polarization-related proteins. (A) Gel blots showing protein expression of Arg-1, iNOS, p-PPARγ and PPARγ in cells with different treatment groups. (B-D) Quantification of (B) Arg-1, (C) iNOS and (D) p-PPARγ based on the western blot assay (n=3). ** P<0.01 compared with M0 group; # P<0.05, ## P<0.01 and $$ P<0.01 compared with M2 group; ▲ P<0.05 and ▲▲ P<0.01 compared with M2 + RSG group. CB, cinobufagin; Arg-1, arginase 1; iNOS, inducible nitric oxide synthase; p-, phosphorylated; PPARγ, peroxisome proliferator-activated receptor γ; RSG, rosiglitazone; ns from M2 group, not statistically significant compared with M0 group; ns from M2 + RSG group, not statistically significant compared with M2 group.

    Article Snippet: After that, primary antibodies against Arg-1 (1:1,000; cat. no. bs-23837R; BIOSS), inducible nitric oxide synthase (iNOS; 1:1,000; cat. no. bs-0162R; BIOSS); phosphorylated (p)-PPARγ (1:1,000; cat. no. bs-3737R; BIOSS), vimentin (1:1,000; cat. no. bsm-33170M; BIOSS), E-cadherin (1:1,000; cat. no. bs-1519R; BIOSS), N-cadherin (1:1,000; cat. no. bs-20623R; BIOSS) and GAPDH (1:3,000; cat. no. 60004-1-Ig; Proteintech Group, Inc.) were incubated with the membrane overnight at 4°C, followed by washing with Tris-buffered saline containing 0.05% Tween 20 and incubation with horseradish peroxidase-conjugated secondary antibodies goat anti-mouse IgG (1:10,000; cat. no. SA00001-1) and goat anti-rabbit IgG (1:10,000; SA00001-2; both from Proteintech Group, Inc.) at room temperature for 1 h. The western blot bands were visualized using an ECL kit (cat. no. PE0010; Beijing Solarbio Science & Technology Co., Ltd.).

    Techniques: Expressing, Western Blot